FISHERIES RESEARCH INSTITUTE,MOA,TAIWAN

This research conducted nutrient component analysis, extraction technology development and various antioxidant capacity analysis of Botryocladia leptopoda, in order to increase the utilization and associated added value of B. leptopoda, while promoting the development of the seaweed industry as well. We found that every 100 g of fresh B. leptopoda contains 94.4 g of water, 3.1 g of ash, 10 Kcal of calories, 0.5 g of protein, and 2.0 g of carbohydrates (including 0.9 g of dietary fiber), but no sugar, trans fat, or saturated fat. In addition to the above, glutamate, glycine, cystine, sodium, potassium, magnesium, calcium, phosphorus, boron, iron, copper, nickel, zinc, manganese, and cobalt are also included. In terms of extraction and application research, we found that the hot extraction group provided better results than the other two groups (the untreated group and the ice extraction group) in terms of total sugar, total phenol, reducing power, ability to scavenge superoxide anion, capacity to capture DPPH free radicals, and ability to chelate ferrous ion. In cell experiments, we found that the crude extract of B. leptopoda did not affect the viability of 3T3-L1 cells, while the crude extract significantly decreased the levels of LDH and ROS (by about 60-70%) in cells treated with D-galactose. The crude extract of B. leptopoda thus has a significant protective effect on 3T3-L1 cells that have been damaged by D-galactose. After the cells treated with D-galactose were subjected to the crude extract of B. leptopoda, their SIRT1 levels were significantly increased, while their MMP2 and MMP9 levels were significantly decreased. The above results indicate that B. leptopoda has good anti-oxidant capabilities when subjected to simple hot extraction, and has the potential to be developed as a natural and safe anti-oxidant material.