anesthetic induction time of 2-phenoxyethanol to juvenile grouperEpinephelus coioides decreased when the concentration of 2-PE becomes high fromthe range 300 to 700 ppm. The recovery time of small size grouper was thelongest at 300 ppm of 2-PE concentration, and 400 ppm in large size grouper. Wecalculated three application concentrations in juvenile grouper for 2-PEtreated. There included the optimal anesthetic concentration was 400 ppm in bothsize groupers. Twenty-four hour's sedative concentrations was 100 and 150 ppm,and 24 h LC50 were 185 and 232 ppm of small size and large size grouper. Themortality of S-2PE grouper is begin over 50% when directly exposed to air in 15minutes. The large size grouper (L-2PE) all below 30 % in 20-30 minutes airexposed. In each size control grouper, their mortality is all below 10% during30 minutes exposed to air.