1 1Eastern Marine Biology Research Center, Fisheries Research Institute 2National Museum of Marine Biology and Aquarium 3Institute of Marine Biodiversity and Evolution, National Dong Hwa University ABSTRACT This study is aimed to understand the artificial propagation, larval culture of Golden trevally (Gnathanodon speciosus) and other relevant materials. The fertilized eggs of Golden trevally are buoyant and semi-transparent. The pelagic eggs had an average diameter of 0.91 ± 0.06 mm (Mean ± SD). The eggs contain a single oil globule which was 0.26 ± 0.01 mm in diameter. The fertilized eggs hatched at 22 h after fertilization (Temperature = 25 ± 0.5 ℃; salinity = 32 ~ 33 psu). The newly hatched larvae were about 2.73 ± 0.10 mm in total length. Larvae (2.81±0.06 mm) at the 3rd day post hatching (DPH) were given the first feeding with rotifers (Brachionus plicatilis). Adding microalgae (Nannochloropsis oculata) can stabilize water quality and nourish rotifers. The 10 DPH fry ware about 4.20 ± 0.28 mm in total length and copepod was used as feeds, and the supply of feedings should be applied at the same time. The 30 DPH fry ware about 13.76 ± 1.48 mm in total length and could be fed with pellet diet. The pattern of band and color of the 100 DPH juvenile (110.70 ± 25.02 mm) were similar to those of the adult fish.