The objective of this study was to develop inbred lines of Taiwanese red tilapia (Oreochromis mossambica × O. nilotica) that could provide genetic resources for commercial production. Using one black mating one white, five out of a total 8 pairs produced F1 progenies. The rate of black occurred from 3.17% to 11.72% in 5 progenies. The progenies from no. 3 that had the lowest number of black tilapia were selected to generate inbred F2. In F2, the body color of progenies was examined at the age of three months. Based on the phenotypic pattern, the body color of 13 progenies were grouped into 7 types as black (BB), strips and patches (RB), spotted (RsB), blotches (RsP), pepper (OrP), orange patches (Wr) and pearl white (WW). The goodness of fit by Chi-square showed that the color pattern was fitted as three pairs of gene genetic mode (p > 0.05). Three inbred lines were then selected as TsB (black), TsR (white) and TsR-1 (white patched with red or orange). After three full-sib mating, the inbreeding coefficient of F5 was 0.6718.