Low temperature storage is currently a common method of reducing the activity of crustaceans to reduce their damage during commercial sales and to ensure the safety of operators. This study investigated the anesthetic response and recovery of mud crab (Scylla serrata) in different low temperature environments. The results indicated that the mud crab would be anesthetized at 12°C and the anesthesia time decreased with the water temperature decreased, but there was no significant difference in recovery time. Exposed to low water temperature for a long time, the recovery time increased with the temperature decreased. After 60 minutes exposed to 6 °C water temperature, the mud crabs began to die, and the survival rate dropped to 83.3%. After the low-temperature tolerance experiment at 6 °C, the mud crabs were reared at room temperature for 14 days, and deaths were found in two groups at 60 minutes and 120 minutes of exposure, and the survival rates were 83.8% and 66.7%, respectively.