This study sought to understand the reproductive behavior and larviculture of Amphiprion clarkii in artificial environments. Moreover, we hope that the results of this study can be used to reduce the trade in wild populations of this species in the ornamental fish market in the future. The results showed that the adhesive demersal eggs of the species are orange in color and ellipsoidal on the surface, with each egg being about 2.53 ± 0.08 mm (Mean ± SD) in total length and 0.94 ± 0.04 mm in width. The yolks were each about 1.64 ± 0.06 mm long and contained several oil droplets with an average diameter of 0.17 ± 0.04 mm, and the newly hatched larvae were about 4.93 ± 0.06 mm in length. In the initial larval stage, the hatchlings were first fed with rotifers. In addition, microalgae were added to stabilize the water quality and to nourish the rotifers. The feedings should be provided consistently at the same time. On the 20th day after hatching, a pellet diet can be provided to the fish fries. On the 180th day, the average body length of the juveniles was 49.60 ± 10.18 mm, and the band pattern and colors of the juveniles were the same as those of adults.