Mass production of broodstock of Litopenaeus vannamei was conducted in an indoor circulating water system. In the first phase, it lasted 7 months for grow-out culture including over-wintering under density of 20 pcs/m2 in three 18-T FRP tanks, the initial average body weight of shrimp was 10-11 g, and the final body average weight was above 20 g. Growth rate and feed conversion ratio of shrimp in the three tanks were almost the same, growth rate was 0.077, 0.071 and 0.075 g/d (0.74 g/d in average), and feed conversion ratio was 2.18, 2.29 and 2.40 (2.29 in average), respectively. Survival rate was 79.1%, 80.1% and 77.3% (79.0% in average), respectively. 4260 grow-out were obtained in total. During early March to later April, the ovary of several shrimp grew to B mature stage. In the second phase, 1960 shrimp were selected from total with 4260 grow-out shrimps was obtained in the first phase, the stocking density was 20 pcs/m2. Shrimp were kept in three tanks on the second floor and two tanks on the first floor for 4 months. The average growth rate and feed conversion ratio was 0.105 g/d and 2.93 on the second floor, respectively, and 0.072 g/d and 2.95 on the first floor, respectively. The average survival rate was 95.5% on the second floor and 94.8% on the first floor. Total with 1865 brood stocks of 30-38 g body weight was obtained from 5 tanks. There was no mature shrimp in 5 tanks during the culture period, from mid-July to early November. Based on the results, we thought that indoor automatic culture system could increase production per unit and produce mass brood stock.